The present study reports the first DNA analysis of HLA class I and class II alleles in FYROMn autistic subjects. It analysed the HLA-A,-C, -B, DRB1 genotypes of 35 autistic patients, and 98 healthy unrelated FYROMns (control group). HLA DNA typing of class I genes was performed using a Reverse Line Strip method (RLS), and the Sequencing Based Typing method (SBT) was used for typing of class II genes.